Antigen

Antigen Class

TFs and others

Antigen

NCOR1

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

Isogenic HEK293 cells, SUMOylation-defective GR (GR3KR), dex, NCOR1 ChIP

cell line

HEK293

cell type

human embryonal kidney cell line

stably expressing

SUMOylation-defective GR (GR3KR)

treatment

dexamethasone

concentration

100 nM

time

1 h

chip antibody

NCOR1 (Bethyl, A301-145A, lot # 2)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, lysates were clarified from sonicated nuclei, and protein-DNA complexes were isolated with specified antibody. For ATAC-seq, 100 000 isolated nuclei were treated with 2.5 µl Nextera Tn5 Transposase from Nextera kit. For ChIP-seq, NEBNext Ultra II DNA Library Prep Kit; for ATAC-seq, Illumina Nextera DNA Library Prep Kit

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

57765133

Reads aligned (%)

82.1

Duplicates removed (%)

7.9

Number of peaks

1133 (qval < 1E-05)

hg19

Number of total reads

57765133

Reads aligned (%)

81.5

Duplicates removed (%)

9.0

Number of peaks

1297 (qval < 1E-05)